Figure 2 stimulus induced brain activity data

This data set shows brain activity in response to an airpuff stimulus applied to the right whiskers. A 2 s, 3 Hz airpuff stimulus is applied to the right whiskers 20 times. Raw .dat files produced by the camera contain wide field images of the mouse brain collected at four different wavelengths. Processed data are included in a .h5 file that contains relative changes in neuronal calcium activity, extracellular acetylcholine, oxyhemoglobin, and deoxyhemoglobin. A video of the mouse face and a recording from an accelerometer placed under the mouse are also included. A trigger file recorded by the data acquisition system shows the timing of measurements and stimuli. This data was used to produce the stimulus response parts of figure 2 in the following article. Doran, P., Fomin-Thunemann, N., Tang, R., Zimmerman, B., Kilic, K., Kura, S., Fisher, H., Chabbot, G., Herbert, J., Jiang, J., Sakadzic, S., Boas, D., Devor, A., Chen, I., & Thunemann, M. (2023). Widefield in vivo imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination. bioRxiv. https://doi.org/https://doi.org/10.1101/2023.11.07.566086do本数据集呈现了小鼠右侧胡须接受气流刺激时的大脑活动。实验向小鼠右侧胡须施加时长2秒、频率3Hz的气流刺激，共重复20次。由摄像系统生成的原始.dat文件包含了四种不同波长下采集的小鼠大脑宽场成像图。处理后的数据存储于.h5文件中，该文件包含神经元钙活动、细胞外乙酰胆碱、氧合血红蛋白以及去氧血红蛋白的相对变化量。数据集还附带小鼠面部的视频录像，以及安装于小鼠下方的加速度计的记录数据。数据采集系统记录的触发文件可用于标注各项测量与刺激的时间节点。本数据集用于生成下述论文中图2的刺激响应部分。 Doran, P.、Fomin-Thunemann, N.、Tang, R.、Zimmerman, B.、Kilic, K.、Kura, S.、Fisher, H.、Chabbot, G.、Herbert, J.、Jiang, J.、Sakadzic, S.、Boas, D.、Devor, A.、Chen, I. 与 Thunemann, M.（2023）. 带有两个荧光通道、两个反射通道、单个sCMOS探测器及屏蔽照明的在体宽场成像系统. bioRxiv. https://doi.org/https://doi.org/10.1101/2023.11.07.566086