Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c18_h1930001_CaB. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c18_h1930001_CaB

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snm3C-seq to brain regions to identify epigenomic landcape of the adult human brains

阐明人脑的细胞架构，对于理解其多样功能与复杂动物行为至关重要。本研究对人脑多个区域开展单细胞核甲基化测序（single nucleus methylation sequencing），鉴定得到多类独立细胞簇，并构建了层级分类系统。这些细胞簇涵盖了已报道的主要脑细胞类型及潜在亚型。我们依托该数据集解析了各类细胞簇的表观基因组特征，并明确了每个细胞簇的特异性调控元件。整体实验设计：对脑区开展snm3C-seq测序，以解析成人脑的表观基因组全景。