16S rRNA gene sequencing of human brain microbiome from Alzheimer's disease-affected and non-demented subjects

Human brain microbiomes were characterized using a high-fidelity, pan-domain, species-specific, full-length 16S rRNA amplification method using Pacific Biosciences HiFi long-read circular consensus sequencing. To assess potential post-mortem microbial contamination of the autopsied brain specimen, the difference in microbial abundance between the environmentally-exposed surface (edge) and the non-exposed core of fresh-frozen brain specimens was analyzed.

本研究采用高保真、泛域、物种特异性的全长16S核糖体RNA（16S rRNA）扩增方法，结合太平洋生物科学公司（Pacific Biosciences）的HiFi长读长环形一致序列测序技术，对人类大脑微生物组进行了表征。为评估尸检大脑标本潜在的死后微生物污染，研究人员对新鲜冷冻大脑标本的环境暴露表面（边缘）与未暴露核心区域的微生物丰度差异展开了分析。