The 4C In planta Quantitative Biotinylproteomics Studies Reveal a WInd-Related Kinase 1 (WIRK1) Functioning as An Early Signaling Component in Wind-induced Thigmomorphogenesis and Gravitropism

Wind is one of the most prevalent environmental forces entraining plants to develop various mechano-responses, collectively called thigmomorphogenesis. Largely unknown is how plants transduce these versatile wind force signals downstream to nuclear events and to the development of thigmomorphogenic phenotype or anemotropic response. To identify molecular components at the early steps of the wind drag force signaling, two mechanical signals-regulated phosphoproteins, identified from our previous phosphoproteomic study of Arabidopsis touch response, mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2), were selected for performing in planta TurboID (ID)-based proximity-labeling (PL) proteomics. This quantitative biotinylproteomics was separately performed on MKK1-ID and MKK2-ID transgenic plants, respectively, using the TurboID overexpression transgenics as a universal control. This quantitative biotinylproteomic work successfully identified 11 and 71 MKK1- and MKK2- associated proteins, respectively. The raw data and mascot search results were summarized and uploaded here.

风力是最为普遍的环境作用力之一，可诱导植物产生多种机械响应，这类响应统称为触形态建成（thigmomorphogenesis）。目前尚不清楚植物如何将这些多样的风力信号向下游转导至细胞核事件，以及触形态建成表型或向风响应（anemotropic response）的形成机制。为鉴定风力拖拽信号转导早期步骤中的分子组分，我们从先前开展的拟南芥触响应磷酸化蛋白质组学研究中筛选得到两个受机械信号调控的磷酸化蛋白：丝裂原活化蛋白激酶激酶1（mitogen-activated protein kinase kinase 1, MKK1）与丝裂原活化蛋白激酶激酶2（MKK2），并以其为材料开展基于植物体内TurboID（ID）的邻近标记（proximity-labeling, PL）蛋白质组学研究。本实验分别对MKK1-ID和MKK2-ID转基因植株进行定量生物素标记蛋白质组学分析，以仅过表达TurboID的转基因植株作为通用对照。本定量生物素标记蛋白质组学实验成功鉴定到分别与MKK1、MKK2相关的11种和71种蛋白。原始数据及Mascot搜索结果已汇总并上传至本数据集。