File S1 - Molecular Evolutionary Analysis of the Alfin-Like Protein Family in <i>Arabidopsis lyrata</i>, <i>Arabidopsis thaliana</i>, and <i>Thellungiella halophila</i>
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Figure S1 in File S1. Amino acid sequence alignment for 20 AL proteins by ML methods with bootstrapping analysis (1000 reiterations). The DUF3594 domain and PHD-finger are indicated by yellow and blue boxed letters. The positively selected codon sites are indicated by red arrows. The amino acids in red box display the altered key site as Lee et al [16]. Figure S2 in File S1. Phenotype response of 35S-AtAL7 A. thaliana lines to normal condition (left), 150 mM NaCl (middle), and 200 mM NaCl (right). Seedlings of vector (upper left), 35S-AtAL7-02 (upper right), 35S-AtAL7-04 (bottom left), and 35S-AtAL7-07 (bottom right) transgenic lines were germinated on a 1/2MS agar plate for 3 days, then transferred to another MS agar plate supplemented with 150 mM NaCl (middle) and 200 mM NaCl (right) for 5 days. Figure S3 in File S1. Diagram of AtAL3 and AtAL7 and their T-DNA insertion mutants. Figure S4 in File S1. Phenotype response of A. thaliana AL mutants to normal condition (left) and 150 mM NaCl (right). Seedlings of wild type (upper left), al7-1 (upper right), al7-2 (bottom left), and al3 (bottom right) mutants were germinated on a 1/2MS agar plate for 3 days, then transferred to another 1/2MS agar plate supplemented with 150 mM NaCl (right) and without (left) for 4 days. (DOC)
文件S1中的图S1：针对20种AL蛋白开展的氨基酸序列比对，采用最大似然法（Maximum Likelihood, ML）结合1000次重复的自举检验（bootstrapping analysis）完成。其中DUF3594结构域与PHD锌指结构域分别以黄色与蓝色框标注；正选择密码子位点以红色箭头标示；红色框内的氨基酸对应Lee等[16]报道的突变关键位点。 文件S1中的图S2：35S启动子驱动AtAL7的拟南芥株系在正常培养条件（左侧）、150 mM氯化钠（NaCl）胁迫（中间）及200 mM氯化钠胁迫（右侧）下的表型响应。将空载对照（左上）、35S-AtAL7-02（右上）、35S-AtAL7-04（左下）与35S-AtAL7-07（右下）转基因株系的幼苗在1/2MS琼脂培养基上萌发3天后，转移至添加150 mM氯化钠（中间）与200 mM氯化钠（右侧）的MS琼脂培养基上继续培养5天。 文件S1中的图S3：AtAL3、AtAL7及其T-DNA插入突变体的结构示意图。 文件S1中的图S4：拟南芥AL家族突变体在正常培养条件（左侧）与150 mM氯化钠胁迫（右侧）下的表型响应。将野生型（左上）、al7-1（右上）、al7-2（左下）与al3（右下）突变体的幼苗在1/2MS琼脂培养基上萌发3天后，转移至添加150 mM氯化钠（右侧）与未添加氯化钠（左侧）的1/2MS琼脂培养基上继续培养4天。 (DOC)
