The Hubbard Brook Stream Ecology Record: Algal Biomass (Chlorophyll-a), 2018 - ongoing

The Hubbard Brook Stream Ecology record is a companion dataset to the Hubbard Brook Watershed Stream and Precipitation Chemistry record. The Stream Ecology record started in 2018 and HBWatER collects ecological samples from seven gauged watersheds: Watersheds 1 through 6 and Watershed 9. HBWatER measures algal biomass, aquatic invertebrate emergence, and stream decomposition by measuring (1) chlorophyll-a on tiles and artificial moss, which approximate algal biomass growth on bare rock and bryophyte mats, (2) preserved algal biomass on artificial moss substrates in Lugol’s Iodine solution, (3) aquatic invertebrate emergence on replicate sticky traps placed above the stream, and (4) stream decomposition through leaf litter pack and cotton strip decay. To complement these ecological records, HBWaTER installed light sensors and field cameras to obtain better information about the light and stream environment daily. Three replicate light sensors that take sub-daily measurements of light level intensity are placed at each watershed at the weir pond (full-sun), and two under the canopy (partial shade). Field cameras take daily photos at noon of the stream canopy and the stream channel. While many studies at Hubbard Brook have measured algal biomass, aquatic invertebrates, and stream decomposition, they are scattered in locations across the valley, were performed at non-continuous times, and use various semi-comparable methods. The HBWatER Stream Ecology record was created to address this gap and systematically measure any long-term changes in the organisms living in the stream. The collection of HBWatER samples is currently sustained by Tammy Wooster (Cary IES) and analyses of these samples has been performed by Heather Malcom (Cary IES), Audrey Thellman (Duke), Steve Anderson (Duke), Geoff Wilson (Cary IES), and Adam Rok (Duke). The dataset is curated and maintained by a team of researchers: Chris Solomon (Cary IES), Emma Rosi (Cary IES), and Emily Bernhardt (Duke). Current Financial Support for HBWatER is provided by NSF LTREB #1907683 and the NSF LTER #XXX (I think you mentioned that you know the LTER fund code for this). Data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.

哈伯布鲁克溪流生态数据集（Hubbard Brook Stream Ecology record）是哈伯布鲁克流域溪流与降水化学数据集（Hubbard Brook Watershed Stream and Precipitation Chemistry record）的配套数据集。该溪流生态数据集始于2018年，HBWatER（哈伯布鲁克流域溪流与降水化学数据集）从7个测流流域（1至6号流域及9号流域）采集生态样本。其测定指标涵盖藻类生物量、水生无脊椎动物羽化情况与溪流分解过程，具体测定方式包括：（1）通过基质片与人工苔藓上的叶绿素a（chlorophyll-a）含量，估算裸岩与苔藓垫上的藻类生物量生长状况；（2）测定卢戈碘液（Lugol’s Iodine solution）中保存的人工苔藓基质上的藻类生物量；（3）依托放置于溪流上方的重复粘性诱捕器，监测水生无脊椎动物的羽化过程；（4）通过枯落叶分解袋（leaf litter pack）与棉条降解（cotton strip decay）实验，测定溪流分解速率。为补充上述生态观测记录，HBWatER布设了光传感器与野外相机，以每日获取溪流环境与光照条件的详细信息。每个流域的堰塘（weir pond，全日照环境）处安装3台可开展亚日级光照强度测量的重复光传感器，另有2台布设于林冠下方（半阴环境）。野外相机于每日正午拍摄溪流冠层与河道的影像。尽管哈伯布鲁克地区已有多项研究测定了藻类生物量、水生无脊椎动物与溪流分解过程，但这些研究分布于山谷各处、观测时间不连续，且采用了多种半可比的实验方法。HBWatER溪流生态数据集旨在填补这一研究空白，系统性监测溪流生物群落的长期动态变化。目前HBWatER样本的采集工作由卡里生态系统研究所（Cary IES）的Tammy Wooster负责开展，样本分析工作由卡里生态系统研究所的Heather Malcom、Geoff Wilson，以及杜克大学（Duke）的Audrey Thellman、Steve Anderson与Adam Rok共同完成。本数据集由研究团队Chris Solomon（卡里生态系统研究所）、Emma Rosi（卡里生态系统研究所）与Emily Bernhardt（杜克大学）进行管理与维护。HBWatER当前的经费支持来自美国国家科学基金会长期环境生物学研究资助项目（NSF LTREB #1907683）及美国国家科学基金会长期生态研究网络（NSF LTER #XXX，原文提及已知该项目的LTER资助代码）。本数据集的采集工作作为哈伯布鲁克生态系统研究（Hubbard Brook Ecosystem Study, HBES）的一部分开展。HBES是设于哈伯布鲁克实验林的协作研究项目，该实验林由美国农业部林务局（USDA Forest Service）北部研究站运营维护。