beta-glucan-induced trained immunity protects against Leishmania braziliensis infection: a crucial role for IL-32. beta-glucan-induced trained immunity protects against Leishmania braziliensis infection: a crucial role for IL-32

We reported the first definitive characterization of the role of IL-32 in the trained phenotype, the results of which improve our understanding of the molecular mechanisms governing trained immunity and Leishmania infection control. Overall design: Examination of the influence of IL32 rs4786370 SNP in the gene expression in bone marrow granulocyte macrophage progenitors (GMP) of healthy volunteers prior and on day 0 and day 90 (M3) post BCG vaccination. GMPs were identified as Lin-CD34+CD38+CD45RA+CD10- events and purified using FACS.

本研究首次明确表征了白细胞介素-32（IL-32）在训练表型中的作用，相关研究结果有助于加深我们对调控训练免疫与利什曼原虫感染控制的分子机制的理解。整体设计：检测健康志愿者卡介苗（BCG）接种前、接种当日（第0天）及接种后第90天（M3）的骨髓粒细胞巨噬细胞祖细胞（GMP）中，IL32 rs4786370单核苷酸多态性（SNP）对基因表达的影响。本研究中通过Lin⁻CD34⁺CD38⁺CD45RA⁺CD10⁻ 细胞事件定义并鉴定GMP，并采用荧光激活细胞分选术（FACS）对其进行纯化。