Genome-wide profiling of mutant p53 unveils a subset of lncRNAs correlated with colorectal cancer stemness maintenance [ChIP-seq]

RNA-sequencing and ChIP-sequencing were used to trace the lncRNAs regulated by p53-R273H in HCT116 endogenous p53 point mutant cell lines. We set out to analyze the differentially expressed lncRNAs in p53-R273H colorectal cancer cell lines compared to wildtype control cell lines (wildtype cell lines as reference) by RNA-seq combined with ChIP-seq. RNA-seq profiling contained three replicates including p53-R273H mutant cell lines and wildtype control cell lines respectively. ChIP-seq profiling contained two replicates including input samples and ChIP samples both of p53-R273H mutant cell lines and wildtype control cell lines.

本研究采用RNA测序（RNA-sequencing）与染色质免疫共沉淀测序（ChIP-sequencing）技术，对HCT116内源p53点突变细胞系中受p53-R273H调控的长链非编码RNA（lncRNAs）进行追踪分析。本研究旨在联合RNA-seq与ChIP-seq技术，对比p53-R273H突变结直肠癌细胞系与野生型对照细胞系（以野生型细胞系作为参照），分析二者间的差异表达lncRNAs。RNA-seq表达谱分析共设置3次生物学重复，分别包含p53-R273H突变细胞系与野生型对照细胞系的样品。ChIP-seq结合谱分析共设置2次生物学重复，样品涵盖p53-R273H突变细胞系与野生型对照细胞系的输入样本（input samples）及ChIP样本（ChIP samples）。