Supplementary Table A Sequences of forward and reverse primers (used in the present study).

Supplementary Table B Percent identity/similarity of CLrP with different sp. of Leishmania and Homo sapiens. Figure A Phylogenetic tree of CLrP calculated using phylogeny.fr (phylogeny.lirmm.fr) with default values of parameters. Figure B LRR motif of L. donovani (CLrP) protein, which contains six repeats that are shown here, along the repeats found in different organisms, including Trypanosoma brucei (ESAG8), C. luciliae (C6K3N8), H.sapiens (SKP2_HUMAN), S. cerevisiae (P38285), A. thaliana (AT1G69545) and P. patens (moss) (A9SSK0). Proteins are encoded as uniprot identifier. Figure C (A) rLdCLrP expression in E. coli, purification and elution were done at 300mM of imidazole concentration and separation in 12%SDS PAGE. M: Molecular wt. Markers, Lane 1 Whole cell lysate (WCL) of uninduced E. coli and Lane2: WCL of E. coli induced at 18°C with 1mM IPTG; Lane3,4: purified rCLrP (B) western blot analysis of E. coli (pET28a+ CLrP) using anti-rLdCLrP antibody. M: Molecular mass marker; Lane1: WCL before IPTG induction; Lane2, WCL after IPTG (1mM) induction at 18°C; Lane3: Purified protein; Figure D Fold expression of Actin (as internal control) in different clinical isolates of L.donovani. Figure E Bioinformatic analysis of CLrP (A) Positioning of LRR motif in CLrP,(B) LdCLrP protein superimposed on template LLR containing human ribonuclease (PDB id: 1z7x), (C)LRR motif and LXXLL motif positioning in CLrP.
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补充表B：CLrP与不同利什曼原虫（Leishmania）种及智人（Homo sapiens）的序列同一性/相似性。
图A：基于phylogeny.fr（phylogeny.lirmm.fr）的默认参数构建的CLrP系统发育树。
图B：杜氏利什曼原虫（L. donovani）CLrP蛋白的富含亮氨酸重复序列（Leucine-Rich Repeat, LRR）基序，该蛋白包含6个重复单元，此处展示了其重复序列，并与不同物种中的同源重复序列进行了比对，包括布氏锥虫（Trypanosoma brucei）ESAG8、C. luciliae（UniProt标识符C6K3N8）、智人SKP2_HUMAN（UniProt标识符）、酿酒酵母（S. cerevisiae，UniProt标识符P38285）、拟南芥（A. thaliana，基因名AT1G69545）以及小立碗藓（P. patens，苔藓，UniProt标识符A9SSK0）。所有蛋白均以UniProt（通用蛋白质知识库）标识符进行标注。
图C：(A) 重组杜氏利什曼原虫CLrP（rLdCLrP）在大肠杆菌（E. coli）中的表达、纯化与洗脱流程：采用300mM咪唑浓度进行洗脱，产物经12%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离。M：分子量Marker；泳道1：未诱导大肠杆菌的全细胞裂解液（WCL），泳道2：18℃下以1mM异丙基-β-D-硫代半乳糖苷（IPTG）诱导的大肠杆菌全细胞裂解液；泳道3、4：纯化后的rCLrP。(B) 采用抗rLdCLrP抗体对携带pET28a(+)-CLrP质粒的大肠杆菌进行蛋白质免疫印迹（Western blot）分析：M：分子量Marker；泳道1：IPTG诱导前的全细胞裂解液；泳道2：18℃下以1mM IPTG诱导后的全细胞裂解液；泳道3：纯化后的目的蛋白。
图D：不同临床分离杜氏利什曼原虫菌株中肌动蛋白（Actin，作为内参蛋白）的相对表达倍数。
图E：CLrP的生物信息学分析：(A) CLrP中LRR基序的定位；(B) 以含人核糖核酸酶的LLR结构域蛋白（蛋白质数据银行Protein Data Bank, PDB编号：1z7x）为模板，对LdCLrP蛋白进行结构叠合；(C) CLrP中LRR基序与LXXLL基序的定位。
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