Molecular consequences of Dlx3 deletion in mouse enamel organ as determined by RNASeq analysis. Mus musculus

DLX3 is a homeodomain transcription factor involved in ameloblast differentiation and enamel formation. Mutations in DLX3 in human lead to defects in enamel. However, the downstream targets of Dlx3 transcriptional activity in the enamel organ have not been identified yet. In this study, we compared the transcriptome of enamel organs where Dlx3 has been deleted to control tissues. Overall design: Total RNA was extracted from enamel organs (mandibular incisors) from Dlx3-WT (N=4) and Dlx3-K14cKO (N=4) mice at P10. cDNA libraries were generated using NEBnext and NuGen kits. Sequencing was performed on the HiSeq2000.

DLX3是一种同源域转录因子，参与成釉细胞分化与牙釉质形成。人体内DLX3发生突变可导致牙釉质缺陷。然而，目前尚未明确牙釉质器官中Dlx3转录调控活性的下游靶基因。本研究将Dlx3敲除的牙釉质器官的转录组与对照组织进行了对比分析。实验设计概况：于出生后第10天（P10），从Dlx3野生型（Dlx3-WT，N=4）与Dlx3-K14cKO（N=4）小鼠的牙釉质器官（下颌切牙）中提取总RNA；使用NEBnext与NuGen试剂盒构建cDNA文库，并在HiSeq2000测序平台上完成测序。