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Heterogeneous Cardiac- and Neural Crest-Derived Aortic Smooth Muscle Cells have Similar Transcriptional Changes after TGFβ Signaling Disruption [scRNA-seq-of-human-aortic-media-vascular-cells]. Heterogeneous Cardiac- and Neural Crest-Derived Aortic Smooth Muscle Cells have Similar Transcriptional Changes after TGFβ Signaling Disruption [scRNA-seq-of-human-aortic-media-vascular-cells]

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1067702
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We report single-cell RNA-seq data of different segments from human thoracic aortas Overall design: Root/Asc/Arch/Desc aortas were collected from human donors in operation room, then digested in collagenase and elastase, stained with CD45, CD235a and viability dye, then subjected to FACS and scRNA-seq *************************************************************** Submitter states that missing raw data are being made available for controlled access in dbGaP. ***************************************************************

本研究报道了人类胸主动脉不同节段的单细胞RNA测序(single-cell RNA-seq)数据。实验设计概述:从手术室的人类供体体内采集主动脉根部(Root)、升主动脉(Asc)、主动脉弓(Arch)及降主动脉(Desc)样本,经胶原酶与弹性蛋白酶消化后,使用CD45、CD235a及活细胞染料进行染色,随后通过荧光激活细胞分选(FACS)并开展单细胞RNA测序。 **************************************************************** 提交者说明,缺失的原始数据将通过dbGaP进行受控访问获取。 ****************************************************************
创建时间:
2024-01-22
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