Expression data from nucleus accumbens of rats infused with lentivirus LV-GFP and LV-miR-495 overexpression constructs. Rattus norvegicus

The goal of the study was to determine the effect of lentiviral- mediated overexpression of miR-495 (LV-miR-495) on the levels of gene expression in the nuclues accumbens of rats relative to control rats injected with the empty vector (LV-GFP). We used Rat Gene 2.0 ST Affymetrix expression arrays ( to identify genes whose expression levels were downregulated by overexpression of miR-495. Overall design: Rats were infused with replication deficient lentiviral contructs LV-miR-495 or control LV-GFP (System Biosciences, Inc) using sterotaxic injections. One week post-infusion the animals were sacrificed and the nucleus accumbens microdissection and processed for RNA extraction using Trizol and RNA was used for hybridization on Affymetrix Rat Gene 2.0 ST arrays. We used 3 biological replicates: rats 1, 3 and 9 were infused with control LV-GFP and rats

本研究旨在探究慢病毒（lentiviral）介导的miR-495过表达（LV-miR-495）对大鼠伏隔核（nucleus accumbens）基因表达水平的影响，并以注射空载体（LV-GFP）的大鼠作为对照。我们采用Affymetrix大鼠基因2.0 ST表达芯片，以筛选经miR-495过表达后表达下调的基因。
实验设计概述：通过立体定向注射向大鼠输注复制缺陷型慢病毒构建体LV-miR-495或对照载体LV-GFP（System Biosciences, Inc）。输注一周后处死动物，显微切割获取伏隔核组织，采用Trizol法提取RNA，将RNA用于Affymetrix大鼠基因2.0 ST芯片的杂交实验。本研究设置3个生物学重复：编号1、3、9的大鼠输注对照载体LV-GFP，其余大鼠