Kinase activities in pancreatic ductal adenocarcinoma with prognostic and therapeutic avenues

The aim of this experiment is to unravel PDAC biology and phospho-print based on aberrant kinase activities and proteome alterations. For this we will perform pTYyrIP, Bravo_IMAC, and protein expression data of n=56 tissues encompassing 47 pancreatic ductal adenocarcinoma (PDAC), 5 cholangiocarcinoma, 1 Intraductal Papillary Mucinous Neoplasm (IPMN), 1 pancreatitis and 1 pancreatitis-Pancreatic Intraepithelial Neoplasia (PanIN) tissue. Pancreatitis, PanIN, and IPMN are seen as early events predecessing PDAC. An equiproportional pool of 5 PDAC cell lines (PANC1, SUIT-2, CFPAC-1, HPAC, MIAPACA2) is also taken along.

本实验旨在基于异常激酶活性与蛋白质组改变，解析胰腺导管腺癌（PDAC, Pancreatic ductal adenocarcinoma）的生物学特性及磷酸化谱（phospho-print）。为此，我们将开展pTYyrIP、Bravo_IMAC检测，并获取n=56份组织的蛋白质表达数据，涵盖47例胰腺导管腺癌（PDAC）、5例胆管癌、1例导管内乳头状黏液性肿瘤（IPMN, Intraductal Papillary Mucinous Neoplasm）、1例胰腺炎以及1例胰腺炎伴胰腺上皮内瘤变（PanIN, Pancreatic Intraepithelial Neoplasia）组织。胰腺炎、PanIN以及IPMN被视为胰腺导管腺癌发生的前驱早期事件。此外，本研究还纳入了由5株胰腺导管腺癌细胞系（PANC1、SUIT-2、CFPAC-1、HPAC、MIAPACA2）组成的等比例混合细胞池。