Total RNA sequencing of postnatal male germ cells

We report the whole-transcriptome profile with total RNA sequencing of postnatal male germ cells. Histone modification levels are dynamically controlled during mammalian spermatogenesis. We found that H3K9 demethylases, Jmjd1a and Jmjd1b catalyze H3K9 demethylation in prospermatogonia. Combined loss of Jmjd1 enzymes disturbed prospermatogonia to spermatogonia transition in mice. To examine a role of Jmjd1 in prospermatogonia to spermatogonia transition, we performed RNA-seq and ChIP-seq analyses using postnatal germ cells at P3 and P7. Overall design: mRNA profiles of postnatal male germ cells isolated from testes of a series of Vasa-Cre; Jmjd1; Jmjd1b conditional knockout mice

本研究报道了出生后雄性生殖细胞的总RNA测序（total RNA sequencing）全转录组图谱。组蛋白修饰（histone modification）水平在哺乳动物精子发生过程中受到动态调控。我们发现，H3K9去甲基化酶Jmjd1a与Jmjd1b可在前精原细胞（prospermatogonia）中催化H3K9去甲基化。小鼠体内Jmjd1家族酶的联合缺失会阻碍前精原细胞向精原细胞（spermatogonia）的转化进程。为探究Jmjd1在前精原细胞向精原细胞转化过程中的作用，我们对出生后第3天（P3）及第7天（P7）的雄性生殖细胞开展了RNA测序（RNA-seq）与染色质免疫共沉淀测序（ChIP-seq）分析。实验整体设计：本研究采集了一系列Vasa-Cre; Jmjd1a; Jmjd1b条件性基因敲除小鼠睾丸中分离得到的出生后雄性生殖细胞的mRNA表达谱。