Epithelial Presenilin-1 drives colorectal tumor growth by controlling EGFR-COX2 signalling

We analyzed the role of Presenilin1 (Psen1) during intestinal tumorigenesis. With this aim, transcriptome comparison of mouse tumor organoids derived from Psen1flox Apcmin/+ and Psen1?IEC Apcmin/+ mice was performed. The analysis revealed alteration in the EGFR pathway and in arachidonic acid metabolism. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Psen1 flox and Psen1 ?IEC Apc tumor organoids with and without EGF treatment.

本研究分析了早老素1（Presenilin1，Psen1）在肠道肿瘤发生过程中的作用。为此，本研究对源自Psen1 flox Apcmin/+小鼠与Psen1ΔIEC Apcmin/+小鼠的小鼠肿瘤类器官进行了转录组比较分析。该分析揭示了表皮生长因子受体（Epidermal Growth Factor Receptor，EGFR）通路及花生四烯酸代谢通路发生异常改变。实验整体设计：对分别经表皮生长因子（Epidermal Growth Factor，EGF）处理与未处理的Psen1 flox及Psen1ΔIEC Apc肿瘤类器官的RNA测序（RNA Sequencing，RNA-seq）数据进行比较基因表达谱分析。