Genome-wide maps of ERα-DNA binding events

To identify WT and RNA-binding-domain-mutated (RBDmut) ERα binding sites on DNA, we performed ChIP-seq of FLAG-tagged ERα. The endogenous ERα in MCF7 cells were replaced with equal amount of WT and RBDmut FLAG-tagged ERα, and cells were subjected to the ChIP-seq procedure.

为鉴定DNA上野生型（Wild Type, WT）与RNA结合结构域突变型（RNA-binding-domain-mutated, RBDmut）的雌激素受体α（Estrogen Receptor α, ERα）结合位点，我们对FLAG标签标记的ERα开展了染色质免疫共沉淀测序（ChIP-seq）。我们将MCF7细胞中的内源性ERα替换为等量的野生型与RBDmut FLAG标签标记的ERα，随后对细胞执行ChIP-seq实验流程。