Human intestinal mycobiome in alcoholic liver disease Targeted loci. human gut mycobiome

We used a high throughput, culture-independent approach by sequencing of the ribosomal internal transcribed sequences (ITS) on the Illumina MiSeq platform, using PCR primers containing BITS and B58S3 to target ITS1, in samples collected from alcoholics and non-alcoholic controls to determine the influence of the intestinal fungal mycobiome on progression of alcoholic liver disease.

本研究采用高通量不依赖培养的研究策略，在Illumina MiSeq测序平台上对核糖体内部转录间隔区（ribosomal internal transcribed sequences，ITS）进行测序；实验使用携带有BITS与B58S3序列的PCR引物靶向扩增ITS1区域，对采集自酗酒者与非酗酒对照组的样本进行分析，以探究肠道真菌组对酒精性肝病进展的影响。