Expression data from murine cardiac fibroblasts and endothelial cells following Transverse Aortic Constriction. Mus musculus

Accumulation of activated cardiac fibroblasts plays a key role in heart failure progression. These cells deposit excessive extracellular matrix that leads to mechanical stiffness, myocyte uncoupling and ischemia. To investigate whether two developmentally distinct cardiac fibroblast populations exhibit distinct expression profiles in response to cardiac injury, and therefore might necessitate distinct therapeutic targeting, we performed microarray analysis on FACS sorted cells. Tie2cre lineage traced CFs, non Tie2cre lineage traced cardiac fibroblasts and endothelial cells were isolated from left ventricle of SHAM operated and banded hearts at the onset of fibrosis, one week after surgery. We used microarrays to detail the global programme of gene expression in cardiac fibroblasts and endothelium following pressure overload. Tie2cre lineage traced, non-tie2cre lineage traced fibroblasts and endothelial cells were sorted from left ventricle of 3 SHAM operated and 3 TAC operated adult male Black Swiss mice. Overall design: Tie2cre lineage traced, non-tie2cre lineage traced fibroblasts and endothelial cells were sorted from left ventricle of 3 SHAM operated and 3 Transaortic Constriction (TAC) operated adult male Black Swiss mice for RNA extraction and Affymetrix microarray analysis. Hypertrophy in TAC animals, an lack of hypertrophy in SHAM operated animals, was evaluated by hemodynamic measurements before surgery and one week after surgery. Cells were isolated one week after surgery.

活化心脏成纤维细胞的积累在心力衰竭进展中发挥关键作用。这类细胞沉积过量细胞外基质，可引发心肌机械僵硬度升高、心肌细胞脱耦联及组织缺血。为探究两种发育起源不同的心脏成纤维细胞群在心脏损伤后是否呈现迥异的基因表达谱，进而可能需要采用差异化的治疗策略，我们对经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）获取的细胞开展了微阵列分析。我们于术后1周，即纤维化发生早期，分别从假手术（SHAM）组与主动脉缩窄手术组小鼠的左心室中，分离得到Tie2cre谱系示踪的心脏成纤维细胞、非Tie2cre谱系示踪的心脏成纤维细胞及内皮细胞。我们借助微阵列技术，解析了压力超负荷条件下心脏成纤维细胞与内皮细胞的全局基因表达程序。本次实验的细胞样本取自3只假手术组与3只主动脉弓缩窄（Transaortic Constriction, TAC）手术组的成年雄性Black Swiss小鼠的左心室，分选出上述三类细胞。实验整体设计：从3只假手术组与3只主动脉弓缩窄（TAC）手术组的成年雄性Black Swiss小鼠左心室中分选上述三类细胞，用于RNA提取及Affymetrix微阵列分析。我们通过术前与术后1周的血流动力学测量，确认主动脉缩窄组小鼠出现心肌肥厚，而假手术组小鼠未出现心肌肥厚表现。所有细胞均于术后1周完成分离。