Tead1 regulation of ß-cells

Purpose: The goal of this study is to determine the regulatory role of tead1 in ß-cells by analyzing the transcriptomal changes with Tead1 deletion in ß-cells Methods: Isolated islet mRNA profiles of ß-cell Tead1 KO mice compared to control floxed mice at 1 year of age were assessed by RNA-seq using Illumina Hiseq2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level using the CLC genomic workbench. qRT-PCR validation was performed using SYBR Green assays Conclusions: Our study represents the first detailed analysis of beta cell transcriptomes following Tead1 deletion in beta cells. Overall design: islated islet total RNA was used for libraby prep from 3 pooled samples for each of 2 samples for each genotype.

研究目的：本研究旨在通过分析β细胞（β-cells）中Tead1缺失后的转录组变化，阐明Tead1在β细胞中的调控作用。
研究方法：采集1年龄β细胞特异性Tead1敲除小鼠与同基因型对照floxed小鼠的分离胰岛mRNA表达谱，采用Illumina Hiseq2500平台进行RNA测序（RNA-seq）分析。通过CLC基因组工作台（CLC genomic workbench）在转录本异构体层面分析通过质量过滤的测序读段。采用SYBR Green法完成qRT-PCR验证。
研究结论：本研究为首次针对β细胞中Tead1缺失后的β细胞转录组开展的详尽分析。
整体实验设计：每个基因型设置2组样本，每组样本由3份混合的分离胰岛总RNA用于文库构建。