Cbx3 Maintains Lineage Specificity During Neural Differentiation [RNA-seq]. Mus musculus

Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 family play important roles in development and differentiation. To determine the regulatroy mechanisms of Cbx3 during neural differentiation from ESCs to NPCs, we performed RNA-seq analysis of ESCs or ESC-derived NPCs depleted for Cbx3 or Cbx3-assocatied Mediator subunit Med26. Overall design: ESCs or ESC-derived NPCs were transfected with control siRNA targeting to luciferase or siRNA mediated knockdown of Cbx3 or Med26. RNAs were extracted from control or knockdown group and subjected to library preparation and deep sequencing.

Cbx3（HP1γ）属于异染色质蛋白1（heterochromatin protein 1, HP1）家族成员，在发育与分化过程中发挥关键作用。为阐明Cbx3在胚胎干细胞（embryonic stem cells, ESCs）向神经前体细胞（neural progenitor cells, NPCs）分化过程中的调控机制，我们对敲低Cbx3或Cbx3相关中介体亚基Med26的ESCs及其分化得到的NPCs开展了RNA测序（RNA-seq）分析。
整体实验设计：将ESCs或ESC来源的NPCs分别转染靶向荧光素酶（luciferase）的阴性对照小干扰RNA（small interfering RNA, siRNA），或靶向Cbx3、Med26的siRNA以实现基因敲低；随后从对照组及各敲低组中提取RNA，进行文库构建与深度测序。