Eothenomys miletus research. Eothenomys miletus

In order to investigate the effects of different areas on intestinal bacterial diversity and body mass regulation in Eothenomys miletus from Hengduan mountain regions, and to explore the community structure and diversity of intestinal microflora and their role in body mass regulation. E. miletus was collected from five areas including Deqin (DQ), Xianggelila (XGLL), Lijiang (LJ), Jianchuan (JC) and Dali (DL), we used 16SrRNA sequencing technology combined with physiological and morphological methods to study the intestinal microbiota diversity, abundance and community structure of the intestinal bacteria in winter, and to explore the influence of geographical factors, physiological indicators including food intake, resting metabolic rate (RMR), non-shivering thermogenesisg (NST), neuropeptide Y (NPY), Agouti-Related Protein (AgRP) proopiomelanocortin (POMC), cocaine and amphetamine regulated transcription peptide (CART), and morphological indicators including body mass, body length and other 9 indicators on the intestinal microflora diversity in E. miletus

为探究横断山区大绒鼠（Eothenomys miletus）不同生境区域对其肠道细菌多样性与体重调控的影响，同时阐明肠道菌群的群落结构、多样性特征及其在体重调控中的作用，本研究从德钦（DQ）、香格里拉（XGLL）、丽江（LJ）、剑川（JC）及大理（DL）5个区域采集大绒鼠样本。采用16S rRNA测序技术结合生理与形态学方法，分析冬季肠道菌群的多样性、丰度及群落结构，并探究地理因素、摄食量、静止代谢率（resting metabolic rate, RMR）、非颤抖性产热（non-shivering thermogenesis, NST）、神经肽Y（neuropeptide Y, NPY）、刺豚鼠相关蛋白（Agouti-Related Protein, AgRP）、阿黑皮素原（proopiomelanocortin, POMC）、可卡因与安非他明调控转录肽（cocaine and amphetamine regulated transcription peptide, CART）等生理指标，以及体重、体长等9项形态学指标对大绒鼠肠道菌群多样性的影响。