Stable knockdown of PLAGL2 in hepatocellular carcinoma cells. Stable knockdown of PLAGL2 in hepatocellular carcinoma cells

Pleomorphic adenoma gene like-2 (PLAGL2), along with PLAG1 and PLAGL1, belong to PLAGL gene family of zinc-finger transcription factors, PLAG proteins are highly homologous, and with the structures of the seven C2H2 zinc finger domain on the N-terminal. PLAG proteins recognize similar GC rich consensus DNA-binding sequences, that these family members function as regulators of transcription target genes and pathways. PLAGL2 has been shown to have both oncogenic and tumor suppressor activities, PLAGL2 is mainly consided as a transcription factor, other functions which may contribute to its oncogenic potential are not clear. So it is meaningful to study the role of PLAGL2 in different diseases. To our knowledge, there is no report about the PLAGL2 in the development process of HCC. Overall design: mRNA profile of Hepatocarcinoma cell line HCC-LM3 with shctrl and shPLAGL2 were generated by deep sequencing

多形性腺瘤基因样因子2（Pleomorphic adenoma gene like-2, PLAGL2）与PLAG1、PLAGL1同属于锌指转录因子PLAGL基因家族。PLAG家族蛋白具有高度同源性，且N端均含有7个C2H2型锌指结构域。PLAG家族蛋白可识别相似的富含GC的保守DNA结合序列，该家族成员均作为转录靶基因及信号通路的调控因子发挥功能。已有研究表明PLAGL2兼具致癌活性与抑癌活性，目前其主要被认定为转录因子，而其他可能参与其致癌潜能的功能机制尚不明确。因此，探究PLAGL2在不同疾病中的作用具有重要研究价值。据我们所知，目前尚无关于PLAGL2在肝细胞癌（Hepatocellular carcinoma, HCC）发生发展过程中作用的相关报道。整体实验设计：通过深度测序获取了分别转染sh对照（shctrl）与shPLAGL2的肝癌细胞系HCC-LM3的mRNA表达谱。