filamentous fungi Targeted loci cultured. filamentous fungi

At the Westerdijk Fungal Biodiversity Institute (previously CBS-KNAW), more than 100,000 living strains of yeasts and filamentous fungi are preserved. Information of the publically available strains such as morphological, physiological, molecular, or any additional data that are relevant to work safely and correctly with the strains, are easily accessible via the website www.westerdijkinstitute.nl. The CBS filamentous fungal collection currently has approximately 70,000 strains including ca. 8,000 ex-type strains representing more than 12,000 currently recognized species, with the oldest strains deposited in 1908. Strain identification is normally achieved with the knowledge and methods available at the time of accession. While taxon names of strains other than ex-type strains are updated following modern taxonomic concepts, it has not been possible to re-evaluate the original identification of each strain in the CBS collection for various reasons. For most of the older strains, not all characters necessary for identification are expressed in culture or modern molecular studies have not been conducted to date. The CBS sequencing project is aimed at generating barcode data from two loci ITS and LSU for all strains included in the collection, 1) to validate these barcodes and (re-)assess strain identity, 2) to enhance the value of the collection and 3) to make these data publicly available. In a previous work, we have released over 8,700 sequences of ~4,700 yeast strains that belong to ~1,400 accepted species. In this study, we aim to release over 24,000 sequences of ~15,000 manually validated filamentous fungal strains that belong to ~6500 accepted species for fungal identification.

Westerdijk真菌生物多样性研究所（原CBS-KNAW）保藏有逾100000株活的酵母菌及丝状真菌菌株。公开可获取菌株的相关信息，包括形态学、生理学、分子生物学数据，以及所有与安全、正确操作该类菌株相关的附加数据，均可通过官网www.westerdijkinstitute.nl便捷获取。CBS丝状真菌馆藏目前约有70000株菌株，其中包含约8000株模式菌株（ex-type strain），代表了目前已确认的逾12000个物种，馆藏最古老的菌株可追溯至1908年。菌株鉴定通常基于菌株入库时所采用的知识与方法完成。除模式菌株外，其余菌株的分类单元名称均已按照现代分类学概念进行更新，但由于诸多原因，我们无法对CBS馆藏中每一株菌株的原始鉴定结果进行重新评估。对于多数老旧菌株而言，要么无法在培养条件下表达鉴定所需的全部特征，要么迄今尚未开展现代分子生物学研究。CBS测序项目旨在为馆藏所有菌株生成两个基因位点：内转录间隔区（ITS）与大亚基核糖体RNA基因（LSU）的分子条形码数据，具体目标有三：其一，验证这些条形码序列并（重新）评估菌株身份；其二，提升该馆藏的科研价值；其三，将这些数据向公众开放。此前的研究中，我们已公开了约1400个公认物种所属的约4700株酵母菌的逾8700条序列。本研究计划公开约6500个公认物种所属的约15000株经人工验证的丝状真菌菌株的逾24000条序列，用于真菌鉴定工作。