Transcriptomics analysis of neurogenesis by striatal astrocytes upon Rbpj-K deletion

We achieved local recombination of striatal astrocytes and silenced Notch signalling activity through Rbpj-K depletion. Recombined astrocytes and neural progenitors generated from the striaral glia were FAC sorted and their molecular profile investigated in a scRNAseq experiment performed 5 wpi. Astrocytes and their neurogenic progeny isolated from striata of mice homozygous for the floxed Rbpj gene and subjected to local injection of AAV-Gfap-Cre

本研究实现了纹状体星形胶质细胞（striatal astrocytes）的原位重组，并通过Rbpj-K敲除（Rbpj-K depletion）沉默了Notch信号通路（Notch signalling）的活性。从纹状体胶质细胞生成的重组星形胶质细胞与神经祖细胞（neural progenitors）经荧光激活细胞分选（fluorescence-activated cell sorting, FACS）后，于注射后5周（weeks post injection, wpi）开展单细胞RNA测序（single-cell RNA sequencing, scRNAseq）实验，以探究其分子特征。从携带纯合floxed Rbpj基因（floxed Rbpj gene）的小鼠纹状体中分离的星形胶质细胞及其神经源性子代细胞，经原位注射腺相关病毒-Gfap-Cre（AAV-Gfap-Cre）处理。