Bulk RNA-seq of a time series of hPSCs as they differentiate towards left ventricle cardiomyocytes. Bulk RNA-seq of a time series of hPSCs as they differentiate towards left ventricle cardiomyocytes

We report that both hESCs and hiPSCs can be differentiated towrads left ventricle cardiomyocytes using an optimised protocol and that these cells transit via first heart field progenitors. Overall design: Examination of the transcriptome of two hPSC lines as they differentiate towards left ventricle cardiomyocytes; critical time points were collected

我们在此报道，通过优化的实验方案，可将人类胚胎干细胞（human embryonic stem cells, hESCs）与人类诱导多能干细胞（human induced pluripotent stem cells, hiPSCs）定向分化为左心室心肌细胞，且该分化过程会经由第一心区祖细胞阶段。实验设计概述：对两株人类多能干细胞系向左心室心肌细胞分化过程中的转录组进行检测，并采集了关键时间点的样本。