Fruit flies sampled from the South African highveld and lowveld for the assessment of fruit fly assemblages and Wolbachia presence

The datasets uploaded in this collection include: 1. R script for nmds (nmds_NK_R.R) along with the csv files used (data and design). 2. Two molecular datasets for the extractions. 3. Fruit fly collection data for the highveld and the lowveld. The project aimed to assess the viability of a Wolbachia- driven insect incompatibility technique control method as a means to aid farmers with the challenges posed by fruit flies. This was done through the completion of the following objectives: 1. Establish the species diversity of tephritid flies in two fruit producing regions of South Africa, the highveld and lowveld, based on morphological identification. 2. Assess the intraspecific genetic diversity of tephritid flies from the highveld and lowveld using a mitochondrial cytochrome oxidase 1 gene barcoding approach. 3. Determine the prevalence and diversity of Wolbachia in naturally infected tephritid species from the highveld and lowveld using PCR-sequencing and next generation sequencing.

本合集上传的数据集包含以下内容：1. 用于非度量多维标度分析（Non-metric Multidimensional Scaling, NMDS）的R脚本（nmds_NK_R.R）及其配套使用的csv数据文件（含样本数据与设计文件）。2. 两份用于提取物分析的分子数据集。3. 针对南非高草原区（highveld）与低草原区（lowveld）的实蝇采集数据。本项目旨在评估沃尔巴克氏体（Wolbachia）介导的昆虫不相容害虫防治技术的可行性，以助力农户应对实蝇带来的种植挑战。研究通过完成以下三项目标开展相关工作：1. 基于形态学鉴定方法，明确南非两大水果产区——高草原区与低草原区的实蝇科（Tephritidae）物种多样性。2. 采用线粒体细胞色素氧化酶1基因（mitochondrial cytochrome oxidase 1, COI）条形码技术，评估高草原区与低草原区实蝇的种内遗传多样性。3. 通过PCR测序与下一代测序（next generation sequencing, NGS）技术，测定高草原区与低草原区自然感染实蝇体内沃尔巴克氏体的携带率与种群多样性。