MTG8 interacts with LHX6 to specify cortical interneuron subtype identity

In the study we compared migrating embryonic cortical interneurons from control mouse embryos and ones carryng homozygous deletions of either Mtg8 or Lhx6. The aim was to identify genes that are co-regulated by LHX6 and MTG8. Overall design: RNA was isolated from fluorescent cells that had been sorted from control and mutant embryonic cortices. The cDNA was used to prepare libraries using the Nextera XT kit (Illumina). These were run on a NextSeq 500 sequencer (lllumina). Raw sequence data were adapter and quality trimmed (fastp v0.20.1), aligned to the genome with STAR (v2.7b) and reads per transcript determined with FeatureCounts (v1.6.4).

本研究对比了对照组小鼠胚胎与携带Mtg8或Lhx6纯合缺失的小鼠胚胎来源的迁移性胚胎皮层中间神经元（embryonic cortical interneurons），旨在鉴定受LHX6与MTG8共同调控的基因。
实验整体设计：从对照组与突变体胚胎皮层中分选得到的荧光标记细胞中提取RNA，以其反转录得到的cDNA为模板，使用Illumina公司的Nextera XT建库试剂盒制备文库，随后将文库置于Illumina NextSeq 500测序仪上进行测序。原始测序数据通过fastp v0.20.1完成接头切除与质量修剪，再使用STAR v2.7b比对至参考基因组，并通过FeatureCounts v1.6.4统计每个转录本的测序读段数。