RNA seq of sorted T cell subsets from PBMCs and rectal biopsies from humans and rhesus macaques. RNA seq of sorted T cell subsets from PBMCs and rectal biopsies from humans and rhesus macaques

Memory CD4 T cells facilitate HIV establishment following transmission and contribute to HIV persistence during suppressive antiretroviral therapy (ART). Numerous studies have highlighted the importance of memory CD4 T cells expressing the surface integrin heterodimer, α4β7 in HIV infection. Here, we focused on delineating the molecular characteristics of α4β7+ CD4 T cells and determining how transcriptional profiles of circulating α4β7hi CD4 T cells corresponded to that within the lower GI tract. Overall design: Comparision of transciptome profiles across blood and gut compartment

人类免疫缺陷病毒（Human Immunodeficiency Virus, HIV）传播后，记忆CD4 T细胞可促进病毒感染的建立，并在接受抑制性抗逆转录病毒治疗（antiretroviral therapy, ART）期间维持病毒的持续存在。多项研究已证实，表达表面整合素异二聚体α4β7的记忆CD4 T细胞在HIV感染过程中发挥关键作用。本研究旨在阐明α4β7阳性CD4 T细胞的分子特征，并明确循环中α4β7高表达CD4 T细胞的转录组特征与下胃肠道内同类细胞的转录组特征的对应关系。整体实验设计：对血液与肠道两个组织区域的转录组图谱进行比较分析。