Expression data of spleens from chickens infected with different Newcastle Disease Viruses. Gallus gallus

Genotype VIId NDV is characterized by severe tissue damage in chicken lymphoid organs compared to other virulent strains. However, biological basis of this unusual pathological phenotype is unknown. Host response is associated with pathogenicity of Newcastle Disease Virus (NDV). We aim to determine the contribution of host response to the severe tissue destruction in the lymphoid system caused by genotype VIId NDV. We used microarray analysis to evaluate the global transcriptional response in the spleen of chickens infected with genotype VIId NDV strain JS5/05 and genotype IV NDV Herts/33. Overall design: Chickens were inoculated with JS5/05 or Herts/33 or mock-infected. At day 2 post infection, spleens were isolated from three chickens per group for RNA extraction and hybridization on Affymetrix microarrays. Samples were named as follows: JS5/05 (I4_1_NS,I4_2_NS,I4_3_NS), Herts/33 (H1_NS,H2_NS, H3_NS), control (C1_NS, C2_NS, C3_NS).

VIId型新城疫病毒（Newcastle Disease Virus, NDV）相较于其他强毒株，其典型特征为可对鸡淋巴器官造成严重组织损伤。然而，这种异常病理表型的生物学基础目前仍未明确。宿主应答与新城疫病毒的致病力密切相关，本研究旨在明确宿主应答在VIId型新城疫病毒引发的淋巴系统严重组织损伤中所发挥的作用。
本研究采用基因芯片（microarray）分析技术，对感染VIId型新城疫病毒毒株JS5/05与IV型新城疫病毒毒株Herts/33的鸡脾脏中的全转录组应答情况进行评估。
实验设计概况：将试验鸡分为三组，分别接种JS5/05毒株、Herts/33毒株，以及进行模拟感染（空白对照）。于感染后第2天，从每组3只试验鸡体内采集脾脏组织，用于RNA提取并在Affymetrix基因芯片上进行杂交。样本命名规则如下：JS5/05组样本为I4_1_NS、I4_2_NS、I4_3_NS；Herts/33组样本为H1_NS、H2_NS、H3_NS；对照组样本为C1_NS、C2_NS、C3_NS。