p53 pulses lead to distinct patterns of gene expression albeit similar DNA-binding dynamics

Investigation of mechanism of gene regulation in response to pulses in p53 protein level induced by gamma irradiation. To directly connect p53 pulses with gene expression, we collected time-course ChIP-Seq and RNA-Seq data. Time course p53, H3K27ac ChIP-Seq and RNA-Seq data in MCF7 cells treated with 10Gy gamma irradiation. As well as time course RNA-Seq data in MCF7 cells expressing p53shRNA.

本研究旨在探究γ射线辐照诱导的p53蛋白水平脉冲变化所对应的基因调控机制。为直接建立p53脉冲与基因表达之间的关联，我们采集了时间序列染色质免疫沉淀测序（ChIP-Seq）及RNA测序（RNA-Seq）数据集：包括经10戈瑞γ射线辐照处理的MCF7细胞的时间序列p53、H3K27ac ChIP-Seq与RNA-Seq数据，以及表达p53短发夹RNA（p53shRNA）的MCF7细胞的时间序列RNA-Seq数据。