Transcriptome of Brain-Derived Neurotrophic Factor-treated neurons

Deep RNA sequencing of primary rat cortical neurons treated with Brain-Derived Neurotrophic Factor (BDNF) to determine the whole genome response to BDNF. Additional treatments of JAK/STAT inhibitors also included to determine which BDNF-induced gene expression changes are carried out by JAK/STAT pathway activation. The goal of this study is to understand the BDNF-induced JAK/STAT pathway further in normal neuronal function and also to understand the role it may play in epilepsy development.

本数据集源自一项针对大鼠原代皮层神经元的深度RNA测序（Deep RNA sequencing）实验：实验采用脑源性神经营养因子（Brain-Derived Neurotrophic Factor, BDNF）处理神经元，以探究神经元全基因组对BDNF的应答反应；同时设置JAK/STAT抑制剂（JAK/STAT inhibitor）处理组，旨在明确BDNF诱导的基因表达变化中，哪些是通过JAK/STAT通路（JAK/STAT pathway）激活所介导的。本研究的核心目标为进一步解析正常神经元功能中BDNF诱导的JAK/STAT通路，并阐明该通路在癫痫发生发展过程中的潜在作用。