Time-series of gene expression in female murine ES cells during EpiSC differentiation. Mus musculus

Embryonic stem cells (ESC) are derived from the inner cell mass of the blastocyst in the presence of leukemia inhibitory factor (LIF). In vivo these cells then differentiate into epi stem cells (EpiSC) that can be derived from the Epiblast in presence of Fgf2 and ActivinA. In this study, female ESCs cultured in 2i medium have been differentiated into EpiSC for 3.5 days in vitro by addition of Fgf2 and Activin A. The gene expression profile was analyzed every 4-12 h using mouse exon arrays. Overall design: Mouse Pgk12.1 ES cells were differentiated into EpiSC, samples were taken every 4-12 hours for 84 hours in total

胚胎干细胞（Embryonic stem cells, ESC）可在白血病抑制因子（leukemia inhibitory factor, LIF）存在的条件下，由囊胚的内细胞团分离获得。在体内环境中，此类细胞可分化为外胚层干细胞（Epi stem cells, EpiSC）；外胚层干细胞可在成纤维细胞生长因子2（Fgf2）与激活素A（ActivinA）存在的条件下，从上胚层中分离得到。本研究中，培养于2i培养基中的雌性ESC通过添加Fgf2与ActivinA，在体外诱导分化3.5天以获得EpiSC。每间隔4至12小时采用小鼠外显子芯片对基因表达谱进行分析。实验整体设计：将小鼠Pgk12.1 ES细胞诱导分化为EpiSC，总诱导时长共计84小时，期间每4至12小时采集一次样本。