Genome-wide identification of AP-2a targets in bladder cells by RNA-seq (Control vs AP-2a KD)

TFAP2A (AP-2a) is a member of the transcription factor AP-2 family, functions as a tumor suppressor by regulating the expression of various cancer-related genes; its expression is positively correlated with chemosensitivity and survival of cancer patients. Overall design: SV-HUC-1 cells were infected with a lentivirus expressing scramble shRNA or AP-2a shRNA for 48 h and then the cells were selected with puromycin (1 µg/mL). Cells infected with scramble shRNA (control) and AP-2a shRNA were sequenced by Illumina HiSeq2000.

TFAP2A（AP-2α）属于转录因子AP-2家族成员，可作为抑癌因子，通过调控多种癌症相关基因的表达发挥功能；其表达水平与癌症患者的化疗敏感性及生存预后呈正相关。
整体实验设计：将SV-HUC-1细胞分别感染携带乱序短发夹RNA（short hairpin RNA，shRNA，阴性对照）或AP-2α shRNA的慢病毒，感染48小时后采用嘌呤霉素（1 μg/mL）进行筛选。随后分别对感染乱序shRNA的对照组细胞与感染AP-2α shRNA的实验组细胞进行Illumina HiSeq2000测序。