Saline soil microbiome study biases caused by different DNA extraction protocols

In the present study, we estimated the bias caused by different DNA extraction methods in saline soil microbiome studies, and we evaluated the microbial diversity and community bias caused only by different physical lysis steps. Also, the bias of amplicon sequencing data processing pipeline, which caused by different DNA extraction methods, was estimated. The bioinformatic processing pipeline included quality filtering, alpha diversity, beta diversity, microbial community composition, phylogenetic analysis, and functional prediction.

本研究针对盐渍土微生物组研究中不同DNA提取方法所引发的偏倚进行了评估，同时仅针对不同物理裂解步骤所导致的微生物多样性与群落偏倚开展了评价。此外，本研究还评估了不同DNA提取方法对扩增子测序数据处理流程所引入的偏倚。本次研究所采用的生物信息学处理流程涵盖质量过滤、α多样性分析、β多样性分析、微生物群落组成分析、系统发育分析以及功能预测。