Expression data from human clear-cell renal cell carcinoma cell line 769-P after overexpression of FOXK2

Clear-cell renal cell carcinoma (ccRCC) is one of the most common urological malignant neoplasms. In addition, ccRCC is a highly aggressive cancer with a concomitant poor prognosis. Forkhead box K2 (FOXK2) has been reported to involve in many molecular mechanisms.However, little is known about the role FOXK2 plays in clear-cell renal cell carcinoma. Our previous study showed that FOXK2 mRNA and protein expression were decreased in human ccRCC tissues and suppressed the proliferation of ccRCC cells both in vitro and in vivo. We used microarrays (Affymetrix HTA2.0 Array) to detail the differentially expressed genes after overexpression of FOXK2, and aim to find potential one or more target gene/genes of FOXK2 We conducted the genome-wide transcriptome profiling of human ccRCC cell line 769-P after transfection with pLV-EGFP-FOXK2 (overexpression of FOXK2) and plv-EGFP empty vector (control)

透明细胞肾细胞癌（clear-cell renal cell carcinoma, ccRCC）是最常见的泌尿系统恶性肿瘤之一。ccRCC同时也是一类高侵袭性恶性肿瘤，患者预后极差。叉头框K2（Forkhead box K2, FOXK2）已被报道参与多种分子调控机制，但目前学界对FOXK2在透明细胞肾细胞癌中的生物学作用仍知之甚少。我们的前期研究发现，FOXK2在人类ccRCC组织中的mRNA及蛋白表达水平均显著降低，且可在体内外抑制ccRCC细胞的增殖。本研究采用Affymetrix HTA2.0 Array基因芯片，对FOXK2过表达后的差异表达基因进行详细解析，旨在筛选FOXK2的潜在靶基因。我们对转染pLV-EGFP-FOXK2（FOXK2过表达组）与pLV-EGFP空载体（对照组）的人ccRCC细胞系769-P开展了全基因组转录组分析。