CHD1 and H3K4me3 ChIP-seq in prostate cancer cell line PC-3

Trimethylation of histone H3 at lysine 4 (H3K4me3) is known to be associated with transcriptional activation and CHD1 is known to selectively recognize and bind to H3K4me3 to activate gene transcription. In addition, CHD1 is involved in the maintenance of open chromatin and cooperates with H3K4me3 to control pluripotency of murine embryonic stem cells. To determine the downstream transcriptional targets and pathways of CHD1 and H3K4me3 in PTEN-null PCa cells, ChIP-seq was performed in control versus CHD1 knockdown PC-3 cells. H3K4me3 and CHD1 ChIP-seq was performed in control versus CHD1 knockdown PC-3 cells.

组蛋白H3赖氨酸4三甲基化（H3K4me3）已被证实与转录激活密切相关，CHD1可选择性识别并结合H3K4me3，进而激活基因转录。此外，CHD1参与维持开放染色质结构，并与H3K4me3协同调控小鼠胚胎干细胞的多能性。为探明PTEN缺失型前列腺癌（PCa）细胞中CHD1与H3K4me3的下游转录靶标及调控通路，本研究在对照组与CHD1敲低的PC-3细胞中开展了染色质免疫共沉淀测序（ChIP-seq）实验；同时在上述对照组及CHD1敲低的PC-3细胞中完成了针对H3K4me3与CHD1的ChIP-seq检测。