scRNAseq on tumor-draining lymph nodes (tdLN) migratory DC and CD4+ T cells in primary melanoma-bearing BP (TyrCre-ERT2Tg/0 BrafLSL-V600E/+ Ptenlox/lox) and BPT (TyrCre-ERT2Tg/0 BrafLSL-V600E/+Ptenlox/lox Tslp-/-) mice

Characterization of migratory DC and CD4 T cells in tdLN from primary melanoma of BP and BPT mice Overall design: We performed multiplexed scRNAseq to characterize migDC and CD4 T cells enriched from tdLN of BP and BPT mice. Hashtag oligos (HTO) were used to label 4 populations for multiplexing.

BP及BPT小鼠原发性黑色素瘤肿瘤引流淋巴结（tumor-draining lymph node, tdLN）中迁移性树突状细胞（migratory dendritic cell, migDC）与CD4阳性T细胞的表征
实验设计概述：本研究采用多重单细胞RNA测序（single-cell RNA sequencing, scRNAseq），对BP及BPT小鼠肿瘤引流淋巴结中富集得到的迁移性树突状细胞与CD4阳性T细胞进行表征分析；实验使用标签寡核苷酸（hashtag oligos, HTO）标记4个细胞群以完成多重混样测序。