Relative V2R/Rluc8 expression in live cells.

HEK293FT or COS7 cells were transfected with the indicated V2R/Rluc8 constructs together with β-arrestin2/Venus. Luminescence was measured in triplicate wells after 2 h pre-treatment with 30 mM EnduRen, as part of the extended BRET kinetic assays (Figure 3). Data shown are the mean ± SEM of 3–7 independent experiments. RLU = relative light units. Luminescence intensity in cells not expressing Rluc8 was 30–150 RLU.针对HEK293FT细胞或COS7细胞，将其与指定的V2R/Rluc8重组构建体及β-抑制蛋白2（β-arrestin2）/Venus荧光蛋白共转染。作为拓展型生物发光共振能量转移（Bioluminescence Resonance Energy Transfer, BRET）动力学检测实验（对应图3）的一部分，以30 mM EnduRen预处理细胞2小时后，于3个复孔中检测发光信号。本次展示的数据为3~7次独立实验的均值±标准误（Standard Error of the Mean, SEM）。RLU为相对光单位（Relative Light Units, RLU）。未表达Rluc8的细胞其发光强度为30~150 RLU。