Expression analysis of the effect of overexpression of Cebpb shRNA in mouse 4T1 mammary tumor cells

Investigation of whole genome gene expression level changes in mouse 4T1 mammary tumors expressing Cebpb shRNA, compared to 4T1 tumors expressing control shRNA. Analysis of mouse 4T1 mammary tumors expressing Cebpb shRNA compared to control shRNA are further described in Johansson & Berg et al 2012. A 10 chip study using total RNA recovered from five separate 4T1 tumors expressing Cebpb shRNA and five separate 4T1 tumors expressing control shRNA. All tumors were surgically removed after subcutaneous implantation in syngeneic BALB/c mice two weeks earlier. Each chip measures the expression level of 44,170 genes from Mus Musculus with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

本研究旨在探究表达Cebpb短发夹RNA（short hairpin RNA，shRNA）的小鼠4T1乳腺肿瘤与表达对照shRNA的4T1乳腺肿瘤之间的全基因组基因表达水平变化。针对表达Cebpb shRNA与对照shRNA的小鼠4T1乳腺肿瘤的相关分析，已在Johansson与Berg等人2012年的研究中得到进一步阐述。本研究共使用10张基因芯片，实验所用总RNA分别提取自5株独立的表达Cebpb shRNA的4T1肿瘤，以及5株独立的表达对照shRNA的4T1肿瘤。所有肿瘤均在同基因BALB/c小鼠皮下植入两周后，通过外科手术切除。每张芯片可检测小家鼠（Mus Musculus）的44170个基因的表达水平，每个基因配有14组24聚体探针对（PM/MM），并设置了三倍技术重复。