Gene expression profiling in patients with a kidney transplantation

Microarrays were used to analyze the gene expression in peripheral blood and kidney allograft biopsies from patients with a kidney transplantation to get more insight in the molecular mechanisms underlying the different clinical phenotypes of kidney transplant rejection. 117 peripheral blood samples and 95 kidney allograft biopsies were used for genome-wide gene expression analysis. The updated Banff 2017 classification was used for diagnosis of the samples. Total RNA extracted from blood and biopsies was used to analyze mRNA expression via Affymetrix Human U133 Plus 2.0 arrays.

本研究采用基因芯片技术，对肾移植患者的外周血样本及移植肾活检组织开展基因表达分析，以深入阐明肾移植排斥反应不同临床表型背后的分子机制。本研究共纳入117例外周血样本与95例移植肾活检组织，用于全基因组基因表达分析。所有样本的诊断均采用2017年更新版Banff分类标准。从血液与活检组织中提取的总RNA，通过Affymetrix Human U133 Plus 2.0基因芯片平台完成mRNA表达水平检测。