File S1 - Oligosaccharide Substrate Preferences of Human Extracellular Sulfatase Sulf2 Using Liquid Chromatography-Mass Spectrometry Based Glycomics Approaches

Contains the following files: Figure S1. Normalized decreases for each HS composition assuming one sulfate removal turnover by HSulf2 from Fig. 1. A. DP6 Oligosaccharides, B, DP8 oligosaccharides. Figure S2. Non-reducing end oligosaccharide (from heparin lyase I digestion) profiles before and after HSulf2 digestion. A, DP6 with zero acetyl groups, B, DP6 with one acetyl group, C, DP8 with zero acetyl groups, D, DP8 with one acetyl group. Figure S3. Odd number DP7 profiles (from heparin lyase I digestion) before and after HSulf2 digestion. A, B and C show DP7 with zero, one, and two acetyl group receptively. Figure S4. EICs of 6-O-sulfate containing disaccharides before and after HSulf2 digestion. Figure S5. Controls for BaF32 cell proliferation assay. A. Dose-response plotting of long chain bovine kidney heparan sulfate before and after HSulf2 digestion. B. Reponses of control for the cell proliferation assay. (DOCX)

本数据集包含以下补充文件：
图S1：基于图1中HSulf2介导的单次硫酸酯去除酶周转过程，各HS组分的归一化降低量。A：DP6寡糖；B：DP8寡糖。
图S2：经肝素裂解酶I（heparin lyase I）酶解得到的非还原端寡糖，在HSulf2酶解前后的谱图。A：无乙酰基的DP6；B：含1个乙酰基的DP6；C：无乙酰基的DP8；D：含1个乙酰基的DP8。
图S3：经肝素裂解酶I酶解得到的奇数聚合度DP7谱图，HSulf2酶解前后的对比结果。A、B、C分别对应无乙酰基、含1个乙酰基、含2个乙酰基的DP7。
图S4：含6-O-硫酸酯的二糖在HSulf2酶解前后的提取离子色谱图（Extracted Ion Chromatogram, EIC）。
图S5：BaF32细胞增殖实验（BaF32 cell proliferation assay）的对照组。A：长链牛肾来源硫酸乙酰肝素（heparan sulfate, HS）经HSulf2酶解前后的剂量响应曲线；B：细胞增殖实验的对照响应结果。
本数据集文档格式为DOCX。