Application of the ddPCR duplex assay on different sample matrices.

Target value: MON810 content expressed as a percentage in cp/cp ratio.aTarget value certified by the CRM provider or evaluated from the certified value of another CRM in the same series. bTarget value in cp/cp ratio attributed in the ILC-CRL-GMFF proficiency program. cTarget value estimated by qPCR, using a CRM certified in cp/cp ratio (same as “Average MON810% (qPCR)”).Average MON810% (ddPCR): Average of the MON810 content measured by ddPCR.Bias MON810% (ddPCR): Bias of the average MON810 content measured by ddPCR with the target value.Average MON810% (qPCR): Average of the MON810 content measured by qPCR.Bias MON810% (qPCR): Bias of the MON810 content measured by qPCR with the target certified value.n.a.: not applicable (value determined by qPCR is the target value).