Application of the ddPCR duplex assay on different sample matrices.

Target value: MON810 content expressed as a percentage in cp/cp ratio.aTarget value certified by the CRM provider or evaluated from the certified value of another CRM in the same series. bTarget value in cp/cp ratio attributed in the ILC-CRL-GMFF proficiency program. cTarget value estimated by qPCR, using a CRM certified in cp/cp ratio (same as “Average MON810% (qPCR)”).Average MON810% (ddPCR): Average of the MON810 content measured by ddPCR.Bias MON810% (ddPCR): Bias of the average MON810 content measured by ddPCR with the target value.Average MON810% (qPCR): Average of the MON810 content measured by qPCR.Bias MON810% (qPCR): Bias of the MON810 content measured by qPCR with the target certified value.n.a.: not applicable (value determined by qPCR is the target value).靶值：以拷贝数比（cp/cp）形式表征的MON810含量百分比。 a. 经标准参考物质（Certified Reference Material, CRM）提供者认证的靶值，或由同系列其他标准参考物质的认证值推导得到的靶值。 b. 在ILC-CRL-GMFF能力验证计划中赋值的cp/cp比形式靶值。 c. 采用经cp/cp比认证的标准参考物质，通过定量聚合酶链式反应（quantitative Polymerase Chain Reaction, qPCR）估算得到的靶值（与"平均MON810%（qPCR）"一致）。 平均MON810%（ddPCR）：通过数字聚合酶链式反应（droplet digital PCR, ddPCR）测得的MON810含量的平均值。 MON810%（ddPCR）偏倚：通过ddPCR测得的MON810含量平均值与靶值之间的偏倚。 平均MON810%（qPCR）：通过qPCR测得的MON810含量的平均值。 MON810%（qPCR）偏倚：通过qPCR测得的MON810含量与经认证的靶值之间的偏倚。 n.a.：不适用（经qPCR测定的值即为靶值）。