Human colorectal tumor tissue: Tumor vs. histologically normal tissue

Transcriptional profiling of human colorectal cancer tissues comparing control from histologically normal tissue samples adjacent to the tumors. High throughput bioluminescence imaging, and PET were performed. The overall goal was to determine the differential expression of lncRNA and mRNA between tumor and match normal samples. Two-condition experiment, Tumor vs. Normal Control. Biological replicates: 9 normal tissue control replicates, 9 colorectal tumor samples. Please note that the experiments were performed technically as dual channel (eg, Cy3 and Cy5-labeled samples hybridized to the same array) but processed as single channel. Thus each raw data file contains raw data for both tumor [C] and normal tissue [N] samples, and is linked to the corresponding tumor tissue sample records.

本研究针对人类结直肠癌组织开展转录组谱分析，以肿瘤旁组织学正常的样本作为对照进行对比。实验同步开展了高通量生物发光成像与正电子发射断层显像（PET）检测。本研究的核心目标为解析肿瘤样本与配对正常样本之间长链非编码RNA（lncRNA）及信使RNA（mRNA）的差异表达水平。本实验为双分组对照设计，分为肿瘤组与正常对照组。生物学重复设置为：正常组织对照样本与结直肠癌肿瘤样本各9例。请注意：本实验在技术层面采用双通道模式（例如，将分别标记有Cy3与Cy5的样本杂交至同一基因芯片阵列），但后续数据分析按单通道模式进行处理。因此，每个原始数据文件均同时包含肿瘤样本[C]与正常组织样本[N]的原始数据，并与对应的肿瘤组织样本记录相关联。