Mus musculus strain:c57BL/6 Genome sequencing

It is known that stress can induce cardiovascular dysfunctions, but the underlying mechanisms are still not well elucidated. The present study aimed to test the hypothesis that alterations of DNA methylation in some cardiac genes may be a mechanism. A mouse model of chronic restraint stress (CRS) was used. Cardiac DNA methylation were sequenced respectively by representation bisulfite sequencing (RRBS).

已知应激可引发心血管功能障碍，但其具体潜在机制尚未完全明晰。本研究旨在验证下述假说：部分心脏基因的DNA甲基化修饰改变或许是介导该病理过程的潜在机制之一。本研究采用慢性束缚应激（chronic restraint stress, CRS）小鼠模型，通过代表性亚硫酸氢盐测序（representation bisulfite sequencing, RRBS）分别对心脏组织的DNA甲基化进行了测序分析。