Expression data from anisomycin- and imiquimod-induced dermatitis. Expression data from anisomycin- and imiquimod-induced dermatitis

We provide a new psoriasis mouse model which is induced by p38 activation in skin. We compared the individual transcriptomic profiles of anisomycin and imiquimod-induced dermatitis. We used microarrays to compare the gene expression pattern between anisomycin- and imiquimod- induced dermatitis. Overall design: Total RNAs prepared from the ears of mice treated with anisomycin and IMQ for 5 consecutive days (n = 3) were subjected to the microarray analysis. An amplified sense-strand DNA product was synthesized with the Ambion WT Expression Kit (Life Technologies, Carlsbad, CA), was fragmented and labelled by the WT Terminal Labelling and Controls Kit (Affymetrix, Santa Clara, CA) and was hybridized to a Mouse Gene 1.0 ST Array (Affymetrix).

本研究提供了一种全新的、由皮肤p38激活诱导的银屑病小鼠模型。我们对比了茴香霉素（anisomycin）与咪喹莫特（imiquimod，简称IMQ）诱导性皮炎的个体转录组谱，并通过微阵列技术分析二者的基因表达模式。
整体实验设计：对连续5天经茴香霉素与IMQ处理的小鼠耳部提取的总RNA（每组n=3）进行微阵列分析。具体实验流程如下：使用Ambion WT表达试剂盒（Life Technologies，美国加利福尼亚州卡尔斯巴德市）合成扩增的正义链DNA产物，随后通过WT末端标记与对照试剂盒（Affymetrix，美国加利福尼亚州圣克拉拉市）完成产物的片段化与标记，最终将标记后的产物与小鼠基因1.0 ST阵列（Affymetrix）进行杂交。