miR396e-OsGRF7 module controls rice plant architecture (RNA-Seq). miR396e-OsGRF7 module controls rice plant architecture (RNA-Seq)

Plant-specific growth-regulating factors (GRFs) participate in multiple central developmental processes including root and leaf development, flower and seed formation, plant senescence, and tolerance to stress. While the role of the miRNA-GRFs regulatory module in determining gross morphology, which is one of the most important agronomic traits for crops, have not been comprehensively unraveled yet. Here, we reported that OsGRF7, a target of miR396e and co-activated with OsGIFs, is essential for determining plant architecture in rice. Overexpression of OsGRF7 leads to decreased tiller number, leaf length and leaf angle, reduced plant height and increased grain size, which are mediated by shortened cell length and disordered cell arrangement. Further analyses indicate that OsGRF7 binds the ACRGDA motif in promoters of OsNSP2, OsGASR1 and OsCYP714B1, OsCga1 and OsARF12, which are involved in the synthesis of strigolactones, gibberellins and cytokinins or related to auxin signaling pathway. Our findings establish OsGRF7 as a crucial component in the miR396-OsGRFs/OsGIFs-plant hormone regulatory network that controls rice growth and plant architecture.This dataset records the differential expressed genes between GRF7OE and WT plants. Overall design: Examination of different expressed genes between WT and GRF7OE.

植物特异性生长调节因子（GRFs）参与诸多核心发育过程，涵盖根与叶发育、花与种子形成、植物衰老以及逆境耐受。尽管miRNA-GRFs调控模块在决定作物最重要农艺性状之一的整体形态方面的作用迄今尚未得到全面阐明，本研究报道，作为miR396e靶标并与OsGIFs协同激活的OsGRF7，是调控水稻株型的关键因子。过表达OsGRF7可使水稻分蘖数、叶长与叶夹角降低，株高下降且籽粒尺寸增大，上述表型均由细胞长度缩短与细胞排列紊乱所介导。进一步分析显示，OsGRF7可结合OsNSP2、OsGASR1、OsCYP714B1、OsCga1及OsARF12启动子区域的ACRGDA基序；这些基因分别参与独脚金内酯、赤霉素与细胞分裂素的合成，或与生长素信号通路相关。本研究结果证实，OsGRF7是调控水稻生长与株型的miR396-OsGRFs/OsGIFs-植物激素调控网络中的关键组分。本数据集记录了GRF7过表达（GRF7OE）与野生型（WT）植株间的差异表达基因。实验设计：分析野生型与GRF7过表达植株间的差异表达基因。