Small RNA sequencing of Sclerotinia sclerotiorum mycelial matts lifted from Brassica napus leaves

Sclerotinia sclerotiorum isolate CU8.24 was used to inoculate Brassica napus cultivar AV Garnet leaves using the mycelial matt inoculation technique described in Seifbarghi et al. (2017) (https://pubmed.ncbi.nlm.nih.gov/28356071/). There are 3 replicate in vitro samples, 2 replicate 12 hours post-inoculation samples, and 3 replicate 24 hours post-inoculation samples. The goal was to understand how S. sclerotiorum small RNAs are regulated during infection of B. napus.

本研究使用核盘菌（Sclerotinia sclerotiorum）菌株CU8.24，依照Seifbarghi等（2017）发表于PubMed（https://pubmed.ncbi.nlm.nih.gov/28356071/）的文献中描述的菌丝垫接种技术，对甘蓝型油菜（Brassica napus）品种AV Garnet的叶片进行接种处理。本次实验共设置3组体外重复样本、2组接种后12小时的重复样本以及3组接种后24小时的重复样本。本研究旨在解析核盘菌小RNA在侵染甘蓝型油菜过程中的调控机制。