Comparative transcriptional profiling of two contrasting rice genotypes in response to rice stripe virus infection

During the pathogenesis of virus disease, lots of changes occur in plant hosts including the altered gene expression profiles. Rice stripe virus (RSV) is the type member of Tenuivirus, transmitted by Laodelphax striatellus in a circulative propagative man To investigate the molecular differences in two japonica rice genotypes WuYun3 and KT95-418 in response to RSV infection, Affymetrix rice genome array were used to analyze and compare their transcriptional profiles. Keywords: disease state analysis Nine-day old 20 rice seedlings were exposed to approximately 100 viruliferous small brown planthoppers (Laodelphax striatellus Fall) for two days to be innoculated by RSV. Then they were transplanted in insect-free greenhouse at 25±3C with a daily photope

在病毒病害的致病过程中，植物宿主会发生诸多分子层面的变化，其中包括基因表达谱的改变。水稻条纹病毒（Rice stripe virus, RSV）是纤细病毒属（Tenuivirus）的模式成员，由灰飞虱（Laodelphax striatellus）以循环增殖型传播方式进行传播。为探究两个粳稻（japonica rice）基因型‘武运3号’与‘KT95-418’在应答水稻条纹病毒侵染时的分子差异，本研究采用Affymetrix水稻基因组芯片（Affymetrix rice genome array）对二者的转录组谱进行分析与比较。
关键词：病害状态分析
选取20株9日龄水稻幼苗，将其暴露于约100头带毒灰飞虱（Laodelphax striatellus Fall）中持续2天以完成水稻条纹病毒接种。随后将幼苗移栽至无虫温室中，培养温度为25±3℃，每日光照周期（photope）为