Remodeling rate parameters from six independent experiments.

Inferred remodeling rate parameters from six independent experiments. A “-” indicates unmodeled reactions either due to missing species or low flux in the experiment. The sn1 18:0 to 18:1 rate parameters inferred from two precursor experiments are similar: 0.0673 and 0.07 for the respective 18:0-18:1 and 18:0-22:6 experiments. The sn1 rate parameters of the first row have ratios 1:0.19:029, similar to ratios in the second row 1:0.18:- and third row 1:0.26:0.24. This indicates that the relative reacylation rates of 18:1, 18:0, and 16:1 are consistently ranked across experiments. Likewise, by comparing values within a column, we can determine relative deacylation rates, i.e. 14:118:314:0 at ratios 6.58:3:1 at the sn1 position. Analogous behavior was also observed for the sn2 position (See Supporting Information S1 for details).